Non-Invasive Flow Measurement in Perfusion Systems Using Clamp-On Ultrasonic Technology
Perfusion systems are widely used in upstream bioprocessing to enable continuous cell culture and high-productivity biologics manufacturing. These systems require precise control of media addition, product harvest, and waste removal while maintaining strict sterility.
Application Requirements
Perfusion bioreactor systems operate continuously and demand reliable, real-time monitoring of multiple fluid streams.
Key requirements include:
• Non-invasive flow measurement to maintain sterile conditions in single-use systems
• Accurate monitoring of media feed, harvest, and waste streams
• Stable and repeatable performance for long-duration processes
• Compact instrumentation to fit within space-constrained bioprocess setups
• Ease of automation for integration with process control systems
Within the perfusion process:
• Fresh medium is continuously fed into the bioreactor
• Cells are retained inside the reactor using retention devices
• Product is continuously harvested
• Waste is removed to maintain optimal conditions
To support this continuous operation, flow measurement must be precise, stable, and easily integrated into automated control strategies.
Process Solution
The Brooks Instrument BCU Series Clamp-On Ultrasonic Flow Meter provides a reliable solution for monitoring flow in perfusion systems without compromising sterility.
In operation:
1. Fresh medium enters the bioreactor at a controlled rate
2. The BCU Series flow meter measures flow non-invasively on the tubing
3. Cells are retained within the bioreactor
4. Product is continuously collected
5. Waste is removed from the system
System Configuration
Typical perfusion systems utilize:
• One to four BCU Series ultrasonic flow meters per system, depending on the number of monitored streams (e.g., feed, harvest, waste, recirculation)
Benefits for Upstream Bioprocessing
• Maintains sterile operation with non-invasive sensing
• Improves control of continuous perfusion processes
• Enhances product consistency and yield
• Reduces complexity compared to indirect measurement methods
• Supports scalable and automated bioprocessing workflows
Accurate and reliable flow measurement is essential for successful perfusion-based bioprocessing. The BCU Series Clamp-On Ultrasonic Flow Meter provides a non-invasive, stable, and automation-friendly solution that meets the demanding requirements of upstream single-use systems.
By enabling precise monitoring of critical process streams, the BCU Series supports improved process control, higher productivity, and greater operational efficiency in modern biopharmaceutical manufacturing.
